Ultrastructural demonstration of terminal deoxynucleotidyl transferase (TdT)
نویسندگان
چکیده
منابع مشابه
Histochemical demonstration of terminal deoxynucleotidyl transferase in leukemia.
White blood cells from 22 patients with leukemia and lymphoma were studied for the presence of terminal deoxynucleotidyl transferase with a peroxidase-antiperoxidase method. The enzyme was detected in leukemic cells of 5 patients with acute lymphoblastic leukemia and 1 patient with chronic myelogenous leukemia, whereas 16 patients with different forms of leukemia or lymphoma were negative for t...
متن کاملDemonstration of terminal deoxynucleotidyl transferase in thymocytes by immunofluorescence.
The cellular and subcellular distribution of terminal deoxynucleotidyl transferase (DNA nucleotidylexotransferase; nucleosidetriphosphate:DNA deoxynucleotidylexotransferase, EC 2.7.7.31) in thymocytes and peripheral lymphocytes from rat, mouse, and calf was studied by immunofluorescence using rabbit antiserum to homogeneous transferase from calf. Terminal transferase was readily detected in app...
متن کاملMurine Terminal Deoxynucleotidyl Transferase
Terminal deoxynucleotidyl transferase (TdT) 1 is an enzyme which has the unique property of polymerizing deoxyribonucleotides onto a primer in the absence of a template (1-4) . Chang (4) first reported that TdT is present only in the thymus among various avian and mammalian tissues examined . Her finding was extended to humans by McCaffrey et al . (5), who found that the enzyme was restricted t...
متن کاملDetection of terminal deoxynucleotidyl transferase.
The recently published study of peripheral blood lymphocyte subpopulations in patients with acute immunodeficiency syndrome, by Murray et al,’ may have contributed additional insight into normal T cell ontogenesis. However, the data on terminal transferase (TdT) must be interpreted with caution. It is stated that TdT levels are especially elevated in acute lymphocytic leukemia (ALL) of T cell o...
متن کاملTrack detection on the cells exposed to high LET heavy-ions by CR-39 plastic and terminal deoxynucleotidyl transferase (TdT)
Background: The fatal effect of ionizing radiation on cells depends on Linear Energy Transfer (LET) level. The distribution of ionizing radiation is sparse and homogeneous for low LET radiations such as X or γ, but it is dense and concentrated for high LET radiation such as heavy-ions radiation. Material and Methods: Chinese hamster ovary cells (CHO-K1) were exposed to 4 Gy Fe-ion 2000 keV/...
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ژورنال
عنوان ژورنال: Blood
سال: 1981
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v57.2.368.368